STUDIES ON NEW VIRUS ISOLATES RECOVERED IN TRACHEAL ORGAN CULTURE

Structure-activity relationships of retinoids in hamster tracheal organ culture.

Structure-activity relationships are summarized for 87 retinoids, using reversal of keratinization in the hamster tracheal organ culture system to measure biological activity. Classes of compounds evaluated include all-trans-retinoic acid and its esters, ring-modified analogs of all-trans-retinoic acid and its esters, side-chain-modified analogs of all-trans-retinoic acid and its esters, analog...

Large-quantity production of chicken embryo tracheal organ cultures and use in virus and mycoplasma studies.

Chicken tracheal organ cultures were made from embryos which were 19 to 20 days old. Transversely cut rings of trachea were placed in screw-capped tissue-culture tubes with Eagle's-N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) medium and incubated in roller drums. The method had advantages over other organ culture systems in that these cultures were prepared in numbers similar to ...

Serologic studies of African Horse-sickness Virus with Emphasis on Neutralizaition Test in Tissue Culture

Replication of murine paramyxoviruses in hamster tracheal organ culture and comparison with standard tissue culture methods.

Replication of Sendai virus, pneumonia virus of mice, and SV5 was investigated in tracheal organ cultures from 2- to 4-day-old and 2- and 4-week-old hamsters, and viral infectivity in tracheal explants was compared with that in tissue culture monolayers. Explants from 2- to 4-day-old hamsters produced higher titers of the three paramyxoviruses, as detected by hemadsorption with guinea pig and m...

Ultrastructural alterations produced by polycyclic aromatic hydrocarbons on rat tracheal epithelium in organ culture.

Ultrastructural alterations of columnar respiratory epithe lium were produced by certain polycyclic aromatic hydrocar bons in suckling rat trachea maintained in organ culture for 11 days. The potent carcinogens 7,12-dimethylbenz(a)anthracene (DMBA) and benzo(a)pyrene and the weakly carci nogenic compounds benz(a)anthracene and 5-fluoro-7,12-dimethylbenz(a)anthracene (F-DMBA) produced cells whos...